HUMIMIC ActSense – An electrifying combination of action and sensation

The HUMIMIC ActSense couples stimulation and sensing functionalities in a single multifunctional system. It therefore allows you to sense and control the electrical properties of your organ cultures.

Product description

While simple TEER measurements have been successfully employed to assess the junction dynamics and barrier integrity of endothelial or epithelial models, the HUMIMIC ActSense additionally carries out electrical impedance spectroscopy techniques to probe the electrical footprint of a cultured tissue, allowing, e.g., the evaluation of the growth, spreading, and differentiation of different cell types at different maturation stages.

The simultaneous recording of the cells’ electrical activity is implemented by 8 differential (16 single ended) sensing sites, which allows the sensing, e.g., of cardiac action potential, for a better understanding and assessment of heart tissue behavior and health. The multiple stimulation profiles can be used, e.g.,  to initially promote the maturation, and eventually pacing the contractions of mature cardiomyocytes.

Functionality specifications

Impedance spectroscopy – TEER

  • 1x channel / 8x channel through multiplexing.
  • Single-frequency (TEER): 96-/24-well.
  • Multi-Frequency measurements (impedance spectroscopy).
  • Frequency Range: 10 Hz-100 kHz.
  • Frequency Resolution: 0.1 Hz.

ECG – EMG – EEG

  • Brain (EEG), muscle (EMG), and heart (ECG) activity recording.
  • 8x differential channel (16x single ended sensing sites).
  • 24-Bit channel resolution: down to micro Volts.
  • Gain: 1x, 2x, 4x, 6x, 8x, 12x, 24x.
  • Data rates: up to 16 ksps.

Stimulation

  • 1x channel / 8x channel through multiplexing.
  • Multiple voltage stimulation profiles (square, sine, train of impulses).
  • Stimulation frequency: 0 Hz - 10 MHz.
  • Cyclic stimulation possible.
  • Gain: 1.5x, 2x, 2.5x, 5x.
  • Attenuation: 1x, 10x, 100x, 1000x.

On-Chip Electrodes

Top: Exemplary setup of HUMIMIC ActSense and HUMIMIC Chip2 24-well. Bottom: Electrode design, functionality and configuration. Click to enlarge image.

The HUMIMIC ActSense can be connected with HUMIMIC Chips featuring multi-electrode array (MEA)-like technology. The electrically conductive material of the integrated electrodes is poly (3,4-ethylenedioxythiophene): poly (sodium4-styrenesulfonate) (PEDOT:PSS), a transparent polymer that can be easily ink-jet printed on plastic materials at a very high throughput. The material is characterized by low thermal noise and superior charge injection when compared to standard metal electrodes, thus resulting in higher signal-to-noise recordings. Moreover, the transparency of the electrodes allow a simultaneous monitoring of the culture by means of standard microscopy techniques.

Request more information

You would like to request contact to a sales representative for more information? Feel free to reach out to us at the following email address: customer@tissuse.com